• Question: How do you pull the DNA from different fruits?

    Asked by DYSH to Sahra on 8 Mar 2017.
    • Photo: Sahra Uygun

      Sahra Uygun answered on 8 Mar 2017:


      Great question! In the lab, we use reagents to isolate DNA in the purest form possible without the DNA-binding proteins so mostly it is not possible to see a lump of DNA in the test tube from the plant.
      But during the science demonstrations to show that fruits indeed have DNA in them, we use a simple experiment outlined here with pictures:

      http://www.funsci.com/fun3_en/dna/dnaen.htm
      or
      http://learn.genetics.utah.edu/content/labs/extraction/howto/

      So the first step is to crush the fruit such as strawberry and make it a pulp to separate cells so that we can use our extraction solution to destroy membranes. The extraction solution include liquid detergent, salt and water.
      After adding the extraction solution to the fruit pulp and mixing them, the next step is to use a filter (like coffee filter) to get rid of the pulp and use the filtered liquid further. Finally adding cold ethanol or rubbing alcohol makes the DNA in the filtered liquid precipitate and become visible.

      This is a simple but cool way to extract DNA.

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